Publications

Comparative effect of supercritical carbon dioxide and high pressure processing on structural changes and activity loss of oxidoreductive enzymes.
Marszałek K., Doesburg P., Starzonek S., Szczepańska J., Woźniak Ł., Lorenzo J.M. et al. (2019). Journal of CO2 Utilization Volume 29.
Two innovative food preservation techniques were applied to two different enzymes, each resulting in an inactivation/decreased activity of the enzymes. The crystallisation fingerprint method was able to differentiate the untreated samples from either of the two treatments. These results are promising and confirm the potential of the method to be applied to a wide range of different products and research questions.

Influence of dewetting on the crystallization behavior of CuCl₂ in the presence of BSA during evaporation in a Petri dish.
Busscher, N., P. Doesburg, G. Mergardt, A. Sokol, J. Kahl, A. Ploeger (2019). Heliyon Volume 5, Issue 1, e01102.
Physics underlying the formation of the crystallization image.

Advanced panel training on visual Gestalt evaluation of biocrystallization images: ranking wheat samples from different extract decomposition stages and different production systems.
Fritz, J., M. Athmann, J-O. Andersen, P. Doesburg, U. Geier, G. Mergardt (2018). Biological Agriculture & Horticulture.
Crystallization images of wheat samples from Biological and Biodynamic cultivation appear to be able to maintain their crystallisation structure better after a stress test than wheat samples of conventional origin.

Systematic Review of Plant-Based Homeopathic Basic Research: An Update.
Ücker, A., S. Baumgartner, A, Sokol, R. Huber, P. Doesburg, T. Jäger (2018). Homeopathy.
Plant-based test systems are frequently used to investigate possible effects of homeopathic preparations. This article reviews publications on such test systems with regard to publication quality, reproducibility and potential for further research.

Examination of specific effects of different homeopathic preparations on cress seedlings with a CuCl2-biocrystallization assay.
Sokol, AM., P. Doesburg, C. Scherr, S. Baumgartner. Proceedings of the XXX GIRI Meeting; 2016 Sep 09-11; Netherland. Int J High Dilution Res. 2016;15(4):10-10.
Article within the framework of a PhD research that describes the extent to which different homeopathic preparations can be distinguished by means of the crystallization method, as this is not possible with conventional compound analysis.

Application of Crystallization with Additives to Cloudy and Clear Apple Juice (2016). Kahl, J., N. Busscher, P. Doesburg, G. Mergardt, F. Will, V. Schulzova, J. Hajslova, A. Ploeger (2016). Food Analytical Methods.
This article researches the effect of apple juice processing (turbid versus clear juice) at the substance level (HPLC) and with respect to structural changes in crystallization images. This project is a first attempt to bridge the gap between the biocrystallisation method and health research.

Standardization and Performance Test of Crystallization with Additives Applied to Wheat Samples
Kahl, J., N. Busscher, G. Mergardt, J-O. Andersen, P. Doesburg, A. Arlai, A. Ploeger (2015). Food Analytical Methods
Described is the standardization of the crystallization procedure for wheat samples from different origins and varieties, including the identification of factors of influence. Comparisons between different laboratories indicate that the method is transferable.

Laboratory intercomparison for biocrystallization (crystallization with additives) applied to different wheat varieties.
Kahl, J., J-O. Andersen, M. Athmann, N. Busscher, P. Doesburg, J. Fritz, U. Geier, G. Mergardt(2015). Elem. Nat.102: 5-13.
This article describes a series of laboratory intercomparisons conducted in five European laboratories. Coded samples from different wheat varieties could be grouped correctly according to variety in all the five laboratories based on a visual evaluation of the crystallization patterns using defined criteria.

Standardization and performance of a visual Gestalt evaluation of biocrystallization patterns reflecting ripening and decomposition processes in food samples (2014).
This manuscript reports on the development and application of a standardized method to visually characterize 'Gestalts', salient, 'meaningful-wholes' in biocrystallization images that have a relationship to plant physiological processes. Herewith a panel succeeded in a correct ranking of biocrystallization images obtained from diverse agricultural products according to the intensity of the degree of 'Ageing' and 'Ripening'. This developed level of evaluation provides a basis towards food quality judgements relating to plant-physiological processes.

BSc thesis J. Zandbergen Wageningen University in collaboration with Crystal Lab (2014):
Characterising the effect of Röntgen-radiation levels on germinating cress seeds by means of the biocrystallization method (download pdf 2.9 Mb)
Thesis carried out in the framework of the project: X-ray detection in the food industry; determining the effect of X-ray radiation on the germination of cress seeds.

Master thesis D. Abel, TU Berlin, in collaboration with Crystal Lab (2013):
Evaluation of picture forming methods compared to standard analytical methods for detection of structural changes caused by different thermal and non-thermal treatments of raw bovine milk (download pdf 8Mb)

Development of a structural image analysis approach on structures from CuCl2 .2H2O crystallization with agricultural products.
Doesburg, P. and A.F.M. Nierop (2013). COMPAG 90 63–67. doi.org/10.1016/j.compag.2012.11.003

Development of a biocrystallisation method for examining effects of homeopathic preparations on germinating cress seeds (download pdf 8Mb)
Baumgartner, S., P. Doesburg, C. Scherr, J-O. Andersen (2012). eCAM doi.org/10.1155/2012/125945.
We developed a cress seedling, crystallisation fingerprint test-system with which we found very strong evidence for specific biological effects of a potentised Tin D30 solution (nominal dilution of 10-30 of the original substance), relative to water D30.

Standardization of the Steigbild Method
Zalecka, A., J. Kahl, P. Doesburg, B. Pyskow, M.A.S. Huber, K. Skjerbaek, A. Ploeger (2010). BAH 27(1):41-57.

Standardization of the biocrystallization method for carrot samples.
Busscher, N., J. Kahl, J.O. Andersen, M.A.S. Huber, G. Mergardt, P. Doesburg, M. Paulsen, A. Ploeger (2010). BAH. 27(1):1-23.

Discrimination between organically and conventionally grown winter wheat farm pair samples using the copper chloride crystallisation method in combination with computerised image analysis.
Szulc, M., J. Kahl, N. Busscher, G. Mergardt, P. Doesburg, A. Ploeger (2010). COMPAG, 74, 218-222.

Evaporation influences on the crystallization of an aqueous dihydrate cupric chloride solution with additives.
Busscher, N., J. Kahl, P. Doesburg, G. Mergardt, A. Ploeger (2010). JCIS, 334(2) 556-562.

Validation of the Visual Evaluation of Biocrystallizations. Development of a reliable and valid instrument for visual evaluation according to ISO-Norms for sensory analyses.
Huber, M., J.-O. Andersen, J. Kahl, N. Busscher, P. Doesburg, G. Mergardt, S. Kretschmer, A. Zalecka, A. Meelursarn, A. Ploeger, D. Nierop, L. van de Vijver, E. Baars (2010). BAH, 27 (1), 25-40.

First tests of standardized biocrystallization on milk and milk products.
Kahl, J., N. Busscher, P. Doesburg, G. Mergardt, M.A.S. Huber, A. Ploeger (2009). European Food Research and Technology, p. 175-178.

Biocrystallisation and Steigbild: Results at Louis Bolk Instituut.
Doesburg, P., M.A.S. Huber (2007). Elemente der Naturwissenschaft. 87:118-123.

Measuring food quality: concepts, methods and challenges: Proceedings seminar Februari 2007.
Vijver, L.P.L. van de, M.A.S. Huber, P. Doesburg, J.H. Nielsen, G. Wyss, J.R. Bloksma, R. Adriaansen-Tennekes, A. Diveky-Ertsey, C. Hoefkens, S.G. Ivanova-Peneva, D. Kusche, S. Manasievska-Simiae, M. Sikora, Katarz (ed.) 2007. Proc. 12-14 Februari 2007, Louis Bolk Instituut, Driebergen.

The capillary dynamolysis method as a characterized tool for crop quality determination.
Zalecka, A., K. Skjerbaek, P. Doesburg, B. Pyskow, M.A.S. Huber, J. Kahl, A. Ploeger (2006). p. 636-637. In European joint organic congress. Odense/DK. 30-31 may 2006.

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